Product Name:
Human Matrix Metalloproteinase 26 (MMP26) ELISA Kit
Cat.#:
JL19495
Regulatory Status:
Alternative:
Matrilysin-2; Endometase
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.15-10ng/mL
Sensitivity:
0.05ng/mL
Specificity:
This kit recognizes Human MMP26 in samples. No significant cross-reactivity or interference between Human MMP26 and analogues was observed.
Sample Type:
tissue homogenates, cell lysates and other biological fluids
Assay Procedure:

Research Area:
Metabolism
Product Datasheets
| MMP26
Matrix metalloproteinase-26 also known as matrilysin-2 and endometase is an enzyme encoded by the MMP26 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein differs from most MMP family members in that it lacks a conserved C-terminal protein domain.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (ng/mL) | 10 | 5 | 2.5 | 1.25 | 0.62 | 0.31 | 0.15 | 0 |
OD | 2.08 | 1.58 | 1.06 | 0.7 | 0.43 | 0.3 | 0.23 | 0.08 |
Corrected OD | 2 | 1.5 | 0.98 | 0.62 | 0.35 | 0.22 | 0.15 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Human MMP26 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Tissue Homogenates(n=8) | 84-101 | 96 |
Cell Lysates(n=8) | 92-105 | 102 |
| Linearity
Samples were spiked with high concentrations of Human MMP26 and diluted with Universal Diluent to produce samples with values within the range of the assay.Tissue Homogenates(n=4) | Cell Lysates(n=4) | ||
1 : 2 | Range (%) | 84-95 | 87-96 |
Average (%) | 91 | 93 | |
1 : 4 | Range (%) | 89-103 | 87-108 |
Average (%) | 94 | 98 |






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